Development of an ADP-ribosylation assay for residual toxicity in C. difficile binary toxin CDTa using automated capillary western blot
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development and implementation of an optimized control strategy for induction machine in an electric vehicle
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15 صفحه اولCharacterization of the enzymatic component of the ADP-ribosyltransferase toxin CDTa from Clostridium difficile.
Certain strains of Clostridium difficile produce the ADP-ribosyltransferase CDT, which is a binary actin ADP-ribosylating toxin. The toxin consists of the binding component CDTb, which mediates receptor binding and cellular uptake, and the enzyme component CDTa. Here we studied the enzyme component (CDTa) of the toxin using the binding component of Clostridium perfringens iota toxin (Ib), which...
متن کاملClostridium difficile binary toxin CDT
Binary toxin (CDT) is frequently observed in Clostridium difficile strains associated with increased severity of C. difficile infection (CDI). CDT belongs to the family of binary ADP-ribosylating toxins consisting of two separate toxin components: CDTa, the enzymatic ADP-ribosyltransferase which modifies actin, and CDTb which binds to host cells and translocates CDTa into the cytosol. CDTb is a...
متن کاملFunctional significance of active site residues in the enzymatic component of the Clostridium difficile binary toxin
Clostridium difficile binary toxin (CDT) is an ADP-ribosyltransferase which is linked to enhanced pathogenesis of C. difficile strains. CDT has dual function: domain a (CDTa) catalyses the ADP-ribosylation of actin (enzymatic component), whereas domain b (CDTb) transports CDTa into the cytosol (transport component). Understanding the molecular mechanism of CDT is necessary to assess its role in...
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Antigen-evoked influx of extracellular Ca 2 1 into mast cells may occur via store-operated Ca 2 1 channels called calcium release–activated calcium (CRAC) channels. In mast cells of the rat basophilic leukemia cell line (RBL-2H3), cholera toxin (CT) potentiates antigen-driven uptake of 45 Ca 2 1 through cAMP-independent means. Here, we have used perforated patch clamp recording at physiological...
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ژورنال
عنوان ژورنال: Journal of Pharmaceutical and Biomedical Analysis
سال: 2020
ISSN: 0731-7085
DOI: 10.1016/j.jpba.2020.113125